Purpose: To analyze the antibacterial effects in vitro of ethyl- cyanoacrylate (EC) tissue adhesive in different application volumes. Methods: Volumes of 4, 6, 8, 10, 12, 14, 25, and 35 μl of EC were applied onto the sur- face of monolayer cultures of Staphylococcus aureus (ATCC25924), Streptococcus pneumoniae (ATCC49619), Escherichia coli (ATCC25922), Pseudomonas aeruginosa (ATCC27853), and Klebsiella pneumoniae (ATCC13883). The diameter of each EC drop was measured, and the area of the circle of EC (formed after its appli- cation onto the monolayer culture) was calculated.
